Abstract: The chemical compositions of the bio-jet fuel from different resources were analyzed and the effect of resources on product properties was discussed, then a comparison between bio-jet fuel and petroleum-based jet fuel was made to find out the composition difference between them. After that, the bio-jet fuel was blended with petroleum-based jet fuel. The chemical compositions as well as the properties of the blends were determined to see the difference in aspect of properties for above two fuels. The comparison data show that the property of bio-jet fuel was fully in compliance with the requirements of ASTM D7566 appendix 2 and the blends was fully in compliance with the requirements of Jet-3.