STUDY ON TRANSESTERIFICATION CATALYZED BY POLYMER COATED ENZYME

Abstract

Abstract: Aiming at the stability and cost of enzyme catalyst in enzymatic reactive distillation technology, Candida antarctica Lipase B (CALB) was immobilized on the macroporous adsorption resin NKA by hydrophobic interaction, and the immobilized lipase NKA-CALB-PEG was prepared by PEGylation. Compared with the commercial immobilized lipase Novozyme435, the stability and cost of NKA-CALB-PEG were significantly improved. The optimal reaction conditions for NKA-CALB-PEG catalytic transesterification of EtAC and BuOH were as follows: the stirring speed was 300 r/min, the amount of catalyst was 10%, the molar ratio of BuOH/EtAC was 1∶1, the temperature was 65 ℃. At 333.15-348.15 K, the experimental data were fitted to obtain the kinetic equation of the reaction. Comparing the experimental values with the calculated results showed that the macro kinetic equation was reasonable.

Key words: immobilized lipase, PEGylation, transesterification, kinetic equation

CLC Number:

References

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