Abstract: A series of Ni-W based catalysts with different NiO content were prepared by incipient wetness method for hydro-deacidification of Suizhong36-1 crude third line vacuum distillate. The effect of operating conditions was investigated. The test results show that the optimum NiO content is around 4.9%. At the conditions of 315℃, 3.2 MPa, hydrogen to oil ratio 300, and volume space velocity 1.5 h-1, the acid value of Suizhong36-1 third line vacuum distillate is reduced to 0.05 mgKOH/g from 3.48 mgKOH/g of feed. A long term stability test for more than 2 000 h is conducted. The results indicate that compared with reference catalyst, the catalyst prepared has a lower cracking degree and is more stable under the moderate conditions.