Abstract: In this paper, a large crystal size TS-1 molecular sieve was synthesized using inorganic silica sol and TiCl3 as silicon source and titanium source respectively. The synthesis of cyclohexanone oxime by ammoximation of cyclohexanone was carried out over the prepared TS-1 molecular sieve in a continuous slurry reactor at 75 oC and WHSV of 6 h-1. The fresh and used TS-1 molecular sieve samples were characterized by XRD, XRF, FT-IR, SEM, TEM and N2 adsorption-desorption methods. It was found that the structure of TS-1 molecular sieve samples prior and post to catalytic reaction was basically unchanged. Within running 190 h, the conversion of cyclohexanone and the selectivity of cyclohexanone oxime maintained around 99.0% and 96.0%, respectively, which indicated that the catalytic activity and stability of the prepared large crystal size TS-1 molecular sieve was similar, or even better than that of TS-1 molecular sieve prepared from organic materials. Furthermore, this TS-1 molecular sieve could be easily separated from the reaction mixture, and after regeneration its catalytic performance was close to that of fresh one.