Abstract: Based on the separation mechanism of high-performance liquid chromatography (HPLC), an analytical method was established for determining acetone content in acetone cyanohydrin and process materials. HPLC method was employed to address the impact of varying pH levels in process materials on acetone measurement results. The chromatographic column used was an XDB-C18 column (4.6 mm × 150 mm × 5 μm), with the mobile phase mass fraction set as A (phosphoric acid aqueous solution)/B (acetonitrile)/C (methanol) = 98:1.5:0.5, at a flow rate of 1.5 mL/min. Single-wavelength detection was performed at 290 nm, with a column temperature of 30°C and an injection volume of 1 μL. Experimental results indicated that the spiked recovery rates for low and high acetone concentrations were 98.33%—99.13% and 101.05%—101.12%, respectively, with a linear range of 0.07%—15% and an RSD of 0.31%. This method is safe, reliable, simple to operate, highly sensitive, and accurate, demonstrating promising application prospects in the chemical industry.

Key words: acetone cyanohydrin, acetone, high-performance liquid chromatography