Abstract: A series of Ni-W/Beta-USY-Al2O3 hydro-upgrading catalysts using USY and different amount of Beta zeolite as acid components were prepared by co-impregnation. The catalysts were characterized by BET, Py-IR and NH3-TPD. The hydro-upgrading performance of the catalysts was investigated in a 100 mL high pressure trickle-bed reactor. The effect of Beta zeolite addition on the acidity of the catalysts with the same amount of USY and the hydroupgrading performance were tested. The results show that the Bronsted/Lewis ratio and total acidity of the catalysts increase with the adding amount of β zeolite rising. Ni-W/Beta-USY-Al2O3 catalysts reveal a high hydroupgrading activity. Under the conditions of 10.0 MPa, H2/oil ratio of 700: 1 and 380 ℃ in upgrading zone, the diesel yield is about 90%, density is lowered 0.1 g/cm3, sulfur content is less than 10 μg/g, cetane number increases 19-22 units and condensation point declines dramatically.