Abstract: TiO2-SiO2 composite support was prepared by sol-gel method，and the supported nickel phosphide catalysts, Ni2P/TiO2-SiO2，were prepared by incipient wetness impregnation and in situ H2 reduction method. The samples were characterized by N2 adsorption-desorption, XRD, TPR， NH3-TPD and FT-IR. Their hydrodenitrogenation (HDN) performance was evaluated in a continuous-flow fixed-bed reactor using quinoline as a model molecule to investigate the effect of the active component loading, Ni/P molar ratio and Ti/Si molar ratio. The results show that TiO2-SiO2 composite support has a larger specific surface area. The Ni2P/TiO2-SiO2 catalyst precursor has a better reducibility, which helps the formation of Ni2P phase. Ni2P phase appears for the Ni2P/TiO2-SiO2 catalyst at n(Ni)/n(P)≤1.0. The optimal HDN activity occurs for the Ni2P/TiO2-SiO2 with Ti/Si molar ratio of 1/2，Ni/P molar ratio of 1.0 and the loading of (NiO+P2O5) of 25%.