Abstract: SAPO-5 molecular sieve having hierarchical pore structure (HI-SAPO-5) was synthesized by one-step hydrothermal crystallization method using 3-(trimethoxysilyl) propyl hexadecyldimethyl ammonium chloride (TPHAC) as mesopore directing surfactant. Samples were analyzed by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), nitrogen adsorption-desorption and pyridine-adsorbed infrared (Py-IR). Characterization results showed that pure HI-SAPO-5 molecular sieve was obtained by introducing THPAC into the synthesis system of normal SAPO-5 molecular sieve (N-SAPO-5). Compared with N-SAPO-5 molecular sieve, HI-SAPO-5 molecular sieve possessed much higher external specific surface area, larger mesopore volume, more suitable acid amount and acid strength. As a result, these advantages were in favor of the mass transfer and conversion of bulky sulfur compounds and polyaromatic hydrocarbons. Evaluation results showed that when coker gas oil was hydrotreated over catalyst containing HI-SAPO-5 molecular sieve, improved hydrodesulfurization, hydrodenitrogenation and hydrodearomatization performance were exhibited. The mass fractions of sulfur and above two-ring aromatic hydrocarbons in the treated oil were 8 μg/g and 1.1 %, respectively.