Abstract: Ti-HMS mesoporous molecular sieves were prepared at ambient temperature by using neutral dodecylamine (DDA) as template and tetraethyl orthosilicate (TEOS) as silicon source, the n(SiO2)/n(TiO2) of which were 20, 40, 80 and 160. These samples were characterized by XRD, FT-IR, SEM and the catalytic performance of Ti-HMS was evaluated by using catalytic oxidation of thiophene as probe reaction. The influences of n(SiO2)/n(TiO2)，reaction time and reaction temperature on the catalytic performance of these samples were studied. Results showed that the prepared samples had typical HMS mesoporous structure, and with spherical particle size of 0.2-0.4 μm. Under the same reaction conditions, Ti-HMS molecular sieve having n(SiO2)/n(TiO2) of 20 exhibited the highest sulfur removal rate; the sulfur removal rate of Ti-HMS having n(SiO2)/n(TiO2) of 160 was higher than those of samples with n(SiO2)/n(TiO2) of 40 and 80, which indicated that the catalytic activity of Ti-HMS molecular sieve was depended on its Ti content and pore size. Under the testing conditions, with the prolonging of reaction time and the increase of reaction temperature, the sulfur removal rate increased.