高浓度柴油降解菌的筛选、鉴定及降解特性研究

石油炼制与化工 ›› 2024, Vol. 55 ›› Issue (3): 118-123.

高浓度柴油降解菌的筛选、鉴定及降解特性研究

王朝辉¹,曹青²,李怿¹,李磊¹,王飞龙¹,白正伟¹,贾苒¹

1. 中石化炼化工程（集团）股份有限公司洛阳技术研发中心
2. 河南科技大学基础医学与法医学院

收稿日期:2023-06-06 修回日期:2023-11-27 出版日期:2024-03-12 发布日期:2024-02-28
通讯作者: 李磊 E-mail:lilei.segr@sinopec.com

SCREENING, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF HIGH CONCENTRATION DIESEL DEGRADING BACTERIA

Received:2023-06-06 Revised:2023-11-27 Online:2024-03-12 Published:2024-02-28
Contact: Lei Li E-mail:lilei.segr@sinopec.com

摘要/Abstract

摘要： 从某石油化工退役场地石油烃污染土壤中筛选得到一株高效柴油降解菌株，经过形态学观察和16S rRNA基因序列分析，鉴定该菌株属于鲍特氏菌属（Bordetella），命名其为Bordetella sp. T3（简称T3）。T3适宜在中温、弱碱性的环境条件下生长，在柴油体积分数为1%、接种量（φ）为2%、温度为30 ℃、pH为8.0的条件下降解柴油，第7天可达89.79%的降解率。T3能够耐受柴油体积分数为10%的高浓度环境，在最适培养条件下培养7 d，其对柴油的降解率为38.57%。降解前后柴油的气相色谱对比结果表明，T3可以有效降解柴油的主要组分（C₁₀～C₂₂），具有良好的石油烃污染物修复应用潜力。

关键词: 鲍特氏菌, 柴油, 筛选鉴定, 生物降解, 降解特性

Abstract: A highly efficient diesel degrading strain was isolated from petroleum hydrocarbon contaminated soil at the decommission-site of a petrochemical plant. The strain was identified as Bordetella by morphological observation and 16S rRNA gene sequence analysis, named Bordetella sp.T3. T3 is suitable to grow in medium temperature and weak alkaline environment. It could degrade diesel oil under the conditions of a diesel volume fraction of 1%, an inoculation amount (φ) of 2%,a temperature of 30 ℃ and a pH of 8.0, and the degradation rate of diesel could reach 89.79% on the 7th day. T3 could tolerate high content of 10% diesel, and the degradation rate of diesel was 38.57% under optimal culture condition for 7 days. The results of degradation spectrogram analysis showed that T3 could effectively degrade the main components (C₁₀-C₂₂) of diesel, and had a good application potential for remediation of petroleum hydrocarbon pollutants.

Key words: Bordetella, diesel, screening and identification, biodegration, degradation characteristics

王朝辉曹青李怿李磊王飞龙白正伟贾苒. 高浓度柴油降解菌的筛选、鉴定及降解特性研究[J]. 石油炼制与化工, 2024, 55(3): 118-123.

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