Abstract: A series of Mo-based catalysts with 5 wt% Mo loading based on different support, including Al2O3, SiO2, ZrO2, MgO and MgAl2O4, were prepared by incipient wetness impregnation method and characterized by XRD，BET，NH3-TPD，H2-TPR. The isobutane dehydrogenation activity tests show that 5Mo/MgAl2O4 catalyst exhibits the maximum single pass yield of isobutene (up to 33 w %) at 560 ℃. The characterization results of XRD, BET, NH3-TPD and H2-TPR reveal that compared with the other supports，Mo/MgAl2O4 possesses relatively large specific surface area which facilitates uniform distribution of Mo species. Besides, appropriate acid and redox properties of the catalysts also contribute to effective activation of isobutane and easy desorption of isobutene. Consequently, relatively high isobutane conversion and isobutene selectivity are achieved.